Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria

نویسندگان

  • Giuseppina Di Giacomo
  • Silvia Campello
  • Mauro Corrado
  • Livia Di Giambattista
  • Claudia Cirotti
  • Giuseppe Filomeni
  • Gabriele Gentile
  • Jianhua Zhang
چکیده

Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm)-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

On the live holotype of the Galápagos pink land Iguana , Conolophus marthae Gentile & Snell , 2009 ( Squamata : Iguanidae ) : is it an acceptable exception ?

The Galápagos pink land iguana, Conolophus marthae Gentile & Snell, 2009 (Squamata: Iguanidae) is the latest example of a species being described without the proper deposition of a preserved onomatophore (name-bearing type specimen) in a taxonomic collection. Differently from other recent similar descriptions, the holotype of Conolophus marthae was marked with a Passive Integrated Transponder, ...

متن کامل

Genetic Evidence of Hybridization between the Endangered Native Species Iguana delicatissima and the Invasive Iguana iguana (Reptilia, Iguanidae) in the Lesser Antilles: Management Implications

The worldwide increase of hybridization in different groups is thought to have become more important with the loss of isolating barriers and the introduction of invasive species. This phenomenon could result in the extinction of endemic species. This study aims at investigating the hybridization dynamics between the endemic and threatened Lesser Antillean iguana (Iguana delicatissima) and the i...

متن کامل

Nitrogen excretion during embryonic development of the green iguana, Iguana iguana (Reptilia; Squamata).

Development within the cleidoic egg of birds and reptiles presents the embryo with the problem of accumulation of wastes from nitrogen metabolism. Ammonia derived from protein catabolism is converted into the less toxic product urea or relatively insoluble uric acid. The pattern of nitrogen excretion of the green iguana, Iguana iguana, was determined during embryonic development using samples f...

متن کامل

Basic ecology of the Oaxacan Spiny-tailed Iguana Ctenosaura oaxacana (Squamata: Iguanidae), in Oaxaca, Mexico.

The Oaxacan Spiny-tailed Iguana Ctenosaura oaxacana is a restricted species to the Isthmus of Tehuantepec in Southern Oaxaca, Mexico. This reptile is one of the less known iguanid species. We census-tracked a population in the South ofNiltepec, Oaxaca, Mexico from May 2010 to April 2011. Throughout one year, a total of 10 line transects were situated and recorded in the study area to determine ...

متن کامل

Spatial Ecology of the Endangered Iguana, Cyclura lewisi, in a Disturbed Setting on Grand Cayman

—West Indian rock iguanas (genus Cyclura: Iguanidae) are among the most endangered lizards in the world, and many species will need to occupy human-modified and -occupied habitats to escape extinction. The Grand Cayman Blue Iguana, Cyclura lewisi, is critically endangered with fewer than 25 wild iguanas remaining. To aid the conservation of this and other iguanas, we investigated the spatial ec...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2015